TA with pH meter

6.) Use your pipette to draw 15cc sample of wine (must) and place in 250 ml Flask or 50 ml Beaker. To draw a sample
into the pipette, apply a vacuum (you may gently suck on the red end) to the end of the pipette when inserted in your
sample. “Do Not Use This Pipette to sample the .2N Sodium Hydroxide”.
7.) Draw 20 cc of water into your pipette and add it your sample.
8.) Fill the syringe with 15-20 cc of .2N Sodium Hydroxide and gently squeeze the solution into the top of your
“Closed” burette. “Caution Sodium Hydroxide is Hazardous”. Avoid all skin contact, if contact occurs wash with
water. For eye contact wash with cool water for 15 minutes and seek medical attention. If swallowed contact your
poison control center immediately.
9.) Place an empty container under the burette (not your wine sample) and slowly open the burette valve (stopcock) and
allow 1-2 ml’s of solution to escape and fill the burette spout. Close the valve.
10.) Record the amount of solution in your burette. You will subtract the amount used from this number to determine
your TA.
11.) Measure the pH of your sample with your pH meter and place your wine sample (flask) under the burette.
12.) With one hand on your wine sample and the other on the burette valve, gently swirl the sample while “SLOWLY”
adding sodium hydroxide.
13.) Stop frequently and measure your pH with your meter. With practice you will be able to hold your pH meter in
your sample while swirling and adding sodium hydroxide.
14.) Titrate until your pH reads 8.2. Slow down as your pH approaches 8. It is easy to over shoot your target.
15.) Record the ending solution level in your burette once you’ve achieved a pH of 8.2 in your sample.
16.) Subtract the recorded starting solution level from the ending solution level to determine your TA. Your TA equals
.1 times the amount of .2N Sodium Hydroxide use to reach a pH of 8.2 in your sample. If you used 5 ml of solution​